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La ipertermia contro il tumore al seno
Ipertermia e cellule di tumore della mammella
La rivista Oncology Report ha pubblicato nel Giugno 2011 uno studio in vitro sugli effetti dell'ipertermia sulle cellule MCF-7 di tumore mammario. Le risultanze sono state di una inibizione dell'attività di invasione delle suddette cellule nonché una soppressione dell'attività angiogenetica.
Effect of hyperthermia on invasion ability and TGF-?1 expression of breast carcinoma MCF-7 cells.
Source
Affiliated Tumor Hospital of Guangzhou Medical College, Guangdong 510095, PR China.
Abstract
The present study aimed to investigate heating-induced alterations of breast cancer cell invasion abilities and the potential mechanisms associated with TGF-?1 expression. MCF-7 cells were heated at 43, 45, 47 and 37 °C for 30 min. In vitro cell invasion ability was evaluated by matrigel invasion assay. The activity of matrix metalloproteinase (MMP)-2/9 was investigated by gelatin zymographic assays. Expression of vascular endothelial growth factor (VEGF) and transforming growth factor-?1 (TGF-?1) was investigated by immunocytochemistry and RT-PCR. Apoptosis was analysed by flow-cytometry. The invasive potential of MCF-7 cells was reduced by heating, and MMP-2/9 secretion and enzymatic activity were suppressed. Furthermore, VEGF and TGF-?1 mRNA and proteins were suppressed by hyperthermia. These results suggest that down-regulation of the expression of TGF-?1, EGF and MMPs by hyperthermia probably accounts for the inhibition of the invasive abilities of MCF-7 cells.
Oncol Rep. 2011 Jun;25(6):1573-9. doi: 10.3892/or.2011.1240. Epub 2011 Mar 29.
