Ipertermia e tumore cerebrale

In questo articolo pubblicato dalla rivista International Journal of Hyperthermia si parla dell'efficacia della ipertermia nell'inibire la crescita e la motilità di cellule di tumore cerebrale.

Int J Hyperthermia. 2015 Sep 14:1-9. [Epub ahead of print]

Electro-hyperthermia inhibits glioma tumorigenicity through the induction of E2F1-mediated apoptosis.

Cha J1,2, Jeon TW1, Lee CG1, Oh ST1, Yang HB1, Choi KJ1, Seo D3,4, Yun I1, Baik IH1, Park KR5, Park YN6, Lee YH1.

Author information

Abstract

PURPOSE:

Modulated electro-hyperthermia (mEHT), also known as oncothermia, shows remarkable treatment efficacies for various types of tumours, including glioma. The aim of the present study was to investigate the molecular mechanism underlying phenotypic changes in oncothermiccancer cells.

MATERIALS AND METHODS:

U87-MG and A172 human glioma cells were exposed to mEHT (42 °C/60 min) three times with a 2-day interval and subsequently tested for growth inhibition using MTS, FACS and microscopic analysis. To obtain insights into the molecular changes in response to mEHT, global changes in gene expression were examined using RNA sequencing. For in vivo evaluation of mEHT, we used U87-MG glioma xenografts grown in nude mice.

RESULTS:

mEHT inhibited glioma cell growth through the strong induction of apoptosis. The transcriptomic analysis of differential gene expression under mEHT showed that the anti-proliferative effects were induced through a subset of molecular alterations, including the up-regulation of E2F1 and CPSF2 and the down-regulation of ADAR and PSAT1. Subsequent Western blotting revealed that mEHT increased the levels of E2F1 and p53 and decreased the level of PARP-1, accelerating apoptotic signalling in glioma cells. mEHT significantly suppressed the growth of human glioma xenografts in nude mice. We also observed that mEHT dramatically reduced the portion of CD133+ glioma stem cell population and suppressedcancer cell migration and sphere formation.

CONCLUSIONS:

These findings suggest that mEHT suppresses glioma cell proliferation and mobility through the induction of E2F1-mediated apoptosis and might be an effective treatment for eradicating brain tumours.